General Information

  • ID:  hor004952
  • Uniprot ID:  Q9TWD6
  • Protein name:  Neuropeptide NPF-1
  • Gene name:  NA
  • Organism:  Leptinotarsa decemlineata (Colorado potato beetle) (Doryphora decemlineata)
  • Family:  NPY family
  • Source:  Animal
  • Expression:  Found in the brain of non-diapausing beetles, but not in diapausing beetles.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Leptinotarsa (genus), Doryphorini (tribe), Chrysomelinae (subfamily), Chrysomelidae (family), Chrysomeloidea (superfamily), Cucujiformia (infraorder), Polyphaga (suborder), Coleoptera (order), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity
  • GO BP:  GO:0007218 neuropeptide signaling pathway
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  ARGPQLRLRF
  • Length:  10(1-10)
  • Propeptide:  ARGPQLRLRF
  • Signal peptide:  NA
  • Modification:  T10 Phenylalanine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Stimulates egg development in L.migratoria, but not in N.bullata. May be involved in the regulation of adult diapause.
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004952_AF2.pdbhor004952_ESM.pdb

Physical Information

Mass: 137416 Formula: C54H92N20O12
Absent amino acids: CDEHIKMNSTVWY Common amino acids: R
pI: 12.8 Basic residues: 3
Polar residues: 1 Hydrophobic residues: 4
Hydrophobicity: -68 Boman Index: -3473
Half-Life: 4.4 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 88
Instability Index: 5829 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  8814784
  • Title:  Insect neuropeptide F (NPF)-related peptides: isolation from Colorado potato beetle (Leptinotarsa decemlineata) brain.
  • PubMed ID:  10098622
  • Title:   Led-NPF-1 stimulates ovarian development in locusts.
  • PubMed ID:  15081426
  • Title:   Diapausing Colorado potato beetles are devoid of short neuropeptide F I and II